Genetically engineered cell culture adapted infectious...

Details Genetically engineered cell culture adapted infectious... Genetically engineered cell culture adapted infectious...

C - Chemistry, Metallurgy

12

N

C12N 15/33 (2006.01) A61K 39/12 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01)

Patent

CA 2298956

The present invention relates to a method for the adaptation of infectious bursal disease viruses (IBDV) to growth in CEF cell culture. Changing the codons for amino acid residues 253 (Gln) and 284 (Ala) to 253 (His) and 284 (Thr) allowed bursa adapted Classical and Variant-E IBDV to grow in CEF cell culture. For GLS IBDV only a change of the codon for amino acid residue 284 was necessary.

La présente invention concerne un procédé pour l'adaptation du virus de la maladie de bursite infectieuse (IBDV) à la croissance en culture de cellules CEF. La modification des codons pour les résidus d'acide aminé 253 (Gin) et 284 (Ala) à 253 (His) et 284 (Thr) a permis aux bourses séreuses adaptées à la E-IBDV classique et variante de croître en culture de cellules CEF. Dans le cas de la GLS IBDV, seulement un changement de codon pour l'acide aminé 284 a été nécessaire.

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