Genetically engineered cell lines, and their uses, in...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/554 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2382910

The present invention relates to genetically engineered cell lines, and to their uses, in particular in processes for screening the developmental or post- developmental neurotoxicity of endogenous or exogenous factors, including drugs and other chemicals, also at low-level exposure, for screening pharmaceutically active molecules useful as drugs for the treatment of pathologies involving an abnormal inhibition or activation of cell death, and for mechanistic studies, the said cell lines being genetically transformed with a nucleotide sequence coding for the p53 protein or a nucleotide sequence coding for the bcl-2 protein, and a nucleotide sequence controlling the level of expression of p53 or bcl-2 proteins. The present invention also relates to kits comprising the said genetically engineered cell lines for carrying out the said processes.

L'invention concerne des lignées cellulaires conçues génétiquement et leurs utilisations, en particulier, dans des procédés de dépistage de la neurotoxicité de facteurs endogènes ou exogènes en cours d'évolution ou à la suite de leur évolution, comprenant des médicaments et d'autres produits chimiques, également à un faible degré d'exposition, de dépistage de molécules pharmaceutiquement actives utilisées en tant que médicaments dans le traitement de pathologies présentant une inhibition ou une activition anormale de la mort cellulaire et pour des études élémentaires, lesdites lignées cellulaires étant génétiquement transformées par une séquence de nucléotide, codant la protéine p53 ou par une séquence de nucléotide codant la protéine bcl-2, et par séquence de nucléotide contrôlant le niveau d'expression de la p53 des protéines bcl-2. L'invention concerne également des lots comprenant lesdites lignées cellulaires conçues génétiquement, afin d'appliquer lesdits procédés.

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