Genetically engineered skin cell types and toxicity assays

Details Genetically engineered skin cell types and toxicity assays Genetically engineered skin cell types and toxicity assays

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/071 (2010.01) C12Q 1/68 (2006.01)

Patent

CA 2400706

Described is an immortalized human skin cell type line (preferably keratinocytes, fibroblasts and/or endothelial cells) stably transfected with a vector, wherein said vector comprises a promoter inducible by environmental hazards and a reporter gene in operative linkage thereto. Preferably, the keratinocyte line is the line HaCaT, the fibroblast line is the line WI-26 and the endothelial cell line is the line LT-2. In addition, a living skin equivalent is described. Furthermore, in vitro toxicity assays making use of these immortalized human skin cell type lines or the skin equivalent are described.

La présente invention concerne une lignée de type de cellule immortalisée de la peau humaine (de préférence des cellules kératinocytes, fibroblastiques et/ou endothéliales) transfectées de manière stable par un vecteur, dans laquelle le vecteur comprend un promoteur inductible par des risques environnementaux et un gène rapporteur en liaison de fonctionnement. De préférence, en ce qui concerne la lignée kératinocyte il s'agit de la lignée HaCaT, la lignée fibroblastique est la lignée WI-26 et la lignée de cellules endothéliales est la lignée LT-2. L'invention concerne également un équivalent de peau vivante. Enfin, l'invention concerne des essais in vitro sur la toxicité mettant en oeuvre lesdites lignées des types de cellules immortalisées de la peau humaine ou l'équivalent de peau.

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