Genetically filtered shotgun sequencing of complex...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2365011

This invention provides methods by which repetitive elements can be selectively removed from genomic libraries made from complex eukaryotic genomes. In particular, the invention relates to affecting the efficiency of recovery of novel genes and regulatory sequences, by use of methylation restrictive hosts.

La présente invention concerne des procédés au moyen desquels des éléments répétitifs peuvent être sélectivement éliminés de banques génomiques constituées à partir de génomes complexes d'eucaryotes. L'invention se rapporte en particulier au changement du rendement de récupération de gènes et de séquences régulatrices nouveaux au moyen d'hôtes de méthylation restrictifs.

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