C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/06 (2006.01) C07K 14/195 (2006.01) C12N 1/21 (2006.01) C12N 9/22 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2638772
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl- tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.
L'invention concerne des couples orthogonaux d'ARNt et de synthétases d'ARNt amino-acylé pouvant introduire l'acide aminé non-naturel phényle séléno-cystéine dans des protéines produites dans des cellules-hôtes eubactériennes telles que E. coli. L'invention a, notamment mais pas exclusivement, pour objet de nouvelles synthétases d'ARNt amino-acylé orthogonales, des polynucléotides encodant les nouvelles molécules de synthétases, des procédés d'identification et de production des nouvelles synthétases, des procédés de production de protéines contenant l'acide aminé non-naturel phényle séléno-cystéine et des systèmes de traduction. L'invention a également pour objet des procédés de production de protéines modifiées (c. à d. de protéines lipidées) par modification ciblée du résidu de phényle séléno-cystéine dans une protéine.
Schultz Peter G.
Wang Jiangyun
Fetherstonhaugh & Co.
The Scripps Research Institute
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