Genetically stable allopolyploid somatic fusion product...

C - Chemistry – Metallurgy – 12 – N

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195/9, 195/1.101

C12N 1/16 (2006.01) C12N 15/00 (2006.01) C12N 15/04 (2006.01) C12P 7/06 (2006.01)

Patent

CA 1199593

INVENTORS GRAHAM GEORGE STEWART INGEBORG RUSSELL CHANDRAKANT JAGJIVAN PANCHAL TITLE GENETICALLY STABLE ALLOPOLYPLOID SOMATIC FUSION PRODUCT USEFUL IN THE PRODUCTION OF FUEL ALCOHOLS ABSTRACT The present invention relates to a stable, allopolyploid, somatic fusion product useful in the fermentation arts and especi- ally in the production of fuel alcohols. More particularly, the present invention relates to a novel allopolyploid yeast strain, Saccharomyces diastaticus NCYC 1460, which is the product of the spheroplast fusion of: (a) a hybrid, diploid strain of Sacch.diastaticus; and (b) a fusion partner which is a polyploid brewing yeast strain of the species Saccharomyces uvarum (carlsber- gensis). The fusion product of the present invention is capable of an en- hanced rate of fermentation and a generally higher degree of final attenuation than is typical of either of the fusion partners. Moreover the fusion product also evidences enhanced osmo- and thermo-tolerances and its multiple, non-allelic DEX gene comple- ment renders the organism well suited to the fermentation of sub- strates containing dextrins and oligosaccharides.

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