Genome mapping of functional dna elements and cellular proteins

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 9/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2530810

The disclosure provides methods of examining the binding of proteins to DNA across a genome (e.g., the entire genome or a portion thereof, such as one or more chromosomes or a chromosome regions). In particular, the disclosure relates to a method of identifying a regulatory region (e.g., a protein or enhancer region) of genomic DNA to which a protein of interest binds. In one aspect, the disclosure looks at tissue related regulation. In another aspect, the disclosure looks at developmental related regulation. In yet another aspect, the disclosure looks at regulation of expression in a particular disease state or disorder.

L'invention concerne des procédés relatifs à l'examen de la constitution de protéines d'ADN dans un génome (par exemple, tout ou partie d'un génome, comme un ou plusieurs chromosomes ou une région de chromosome), et en particulier un procédé d'identification de région régulatrice (par exemple, protéine ou région activatrice) d'ADN génomique auquel se lie une protéine spécifique. Selon un aspect, l'invention concerne une régulation liée au tissu, et selon encore un autre aspect elle concerne une régulation liée au développement. Enfin, selon un troisième aspect, l'invention concerne la régulation de l'expression dans le cas d'une maladie ou d'un trouble spécifique.

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