Genomic editing in zebrafish using zinc finger nucleases

A - Human Necessities – 01 – K

Patent

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Details

A01K 67/027 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2700170

Disclosed herein are methods and compositions for genomic editing of one or more genes in zebrafish, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

L'invention porte sur des procédés et des compositions pour l'édition génomique d'un ou de plusieurs gènes dans un dard-perche, à l'aide de protéines de fusion comportant une protéine à doigt de zinc et un domaine de clivage ou un demi-domaine de clivage. L'invention porte également sur des polynucléotides codant pour lesdites protéines de fusion, ainsi que sur des cellules comportant lesdits polynucléotides et lesdites protéines de fusion.

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