Genomic library of cyanophage s-2l and functional analysis

Details Genomic library of cyanophage s-2l and functional analysis Genomic library of cyanophage s-2l and functional analysis

C - Chemistry, Metallurgy

12

N

C12N 7/00 (2006.01) C07D 473/16 (2006.01) C07K 14/01 (2006.01) C07K 16/08 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2483706

The invention relates to the genome sequence and the nucleotide sequences coding for polypeptides of cyanophage S-2L. According to the invention, the polypeptides include, but are not limited to, polypeptides involved in the synthesis, transcription and replication of purine bases. In particular, determining the genome of cyanophage S-2L is useful for supplying genes which, expressed in recombinant bacteria, enable the synthesis of DNA monomers incorporating base D (2,6 diaminopurine) instead of base A (adenine), thereby producing chemically-remodelled nucleic acids in the bacteria.

L présente invention a pour objet la séquence génomique et des séquences nucléotidiques codant pour des polypeptides du cyanophage S-2L. Les polypeptides décrits dans la présente invention sont, de façon non limitative, des polypeptides impliqués dans la synthèse, la transcription et la réplication des bases puriques. En particulier, la détermination du génome du cyanophage S-2L est un outil utile pour la fourniture de gènes, qui exprimés dans des bactéries recombinantes, permettent la sythèse de monomères d'ADN incorporant la base D (2,6 diaminopurine) au lieu de la base A (adénine) et ainsi de produire des acides nucléiques chimiquement remodelés chez les bactéries.

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