Genomic marker for meat tenderness

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2662547

The invention concerns the use of a genomic marker comprising a nucleotide sequence corresponding to all or part of the nucleotide sequence SEQ ID N°1, or to all or a part of an allele of the nucleotide sequence SEQ ID N°1.and associated with the phenotype relating to the tenderness of the meat from bovines, for the implementation of any identification method for the quantification of DNAJA1 expression level with the aim of selecting and/or sorting ruminant animals of the bovine type which produce tender meat and/or for the implementation of an identification method of tender meats at the moment of slaughter by direct sampling from the carcass. The invention concerns identification methods using the real time RT- PCR technique, the hybridization of labelling cDNA with an oligonucleotide probe and the detection of antigen-antibody complexes.

L'invention concerne l'utilisation d'un marqueur génomique qui comprend une séquence nucléotidique correspondant entièrement ou partiellement à la séquence nucléotidique SEQ ID N°1, ou entièrement ou partiellement à un allèle de la séquence nucléotidique SEQ ID N°1 et qui est associé au phénotype concernant la tendreté de la viande de bovins, pour la mise en oeuvre d'un quelconque procédé d'identification pour la quantification du niveau d'expression de DNAJA1 avec pour objectif de sélectionner et/ou de trier des animaux ruminants du type bovin qui produisent de la viande tendre et/ou pour la mise en oeuvre d'un procédé d'identification de viandes tendres au moment de l'abattage par échantillonnage direct à partir de la carcasse. L'invention concerne des procédés d'identification utilisant la technique RT- PCR en temps réel, l'hybridation d'ADNc de marquage avec une sonde oligonucléotidique et la détection de complexes antigène-anticorps.

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