Genotyping of deafness by oligonucleotide microarray analysis

Details Genotyping of deafness by oligonucleotide microarray analysis Genotyping of deafness by oligonucleotide microarray analysis

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2506601

A method for genotyping a subject with respect to a gene or target nucleic acid sequence associated with a pathological condition, said method comprising contacting an allele specific oligonucleotide immobilized to a solid support with a single-stranded from of RNA or DNA from a subject to be tested labeled directly or indirectly with a reporter molecule capable of giving an identifiable signal under conditions which permit hybridization of single stranded RNA or DNA which is exactly complementary to the immobilized allele specific oligonucleotide but substantially less or no hybridization of non- complementary single-stranded RNA or DNA molecules and then screening for the presence or absence or level of reporter molecule which provides an indicator of the genetic identity of the single-stranded RNA or DNA molecule which in turn provides the genotype of the subject.

L'invention concerne un procédé permettant d'établir le génotype d'un sujet par rapport à un gène ou à une séquence d'acides nucléiques cible associés à un état pathologique. Ce procédé consiste: à mettre en contact un oligonucléotide spécifique de l'allèle immobilisé sur un support solide avec de l'ARN ou de l'ADN simple brin d'un sujet marqué de manière directe ou indirecte par un ligand pouvant donner un signal identifiable dans des conditions qui permettent l'hybridation de l'ARN ou de l'ADN simple brin qui est exactement complémentaire à l'oligonucléotide spécifique de l'allèle immobilisé, mais qui ne permettent pas ou sensiblement pas l'hybridation des molécules d'ARN ou d'ADN simple brin non complémentaires; puis à détecter la présence, l'absence ou le niveau de ligand qui fournit une indication de l'identité génétique de la molécule d'ARN ou d'ADN simple brin, qui à son tour permet d'obtenir le génotype du sujet.

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