Gentamicin-resistance genes and their use as markers

Details Gentamicin-resistance genes and their use as markers Gentamicin-resistance genes and their use as markers

C - Chemistry, Metallurgy

12

N

195/1.17, 195/1.

C12N 15/12 (2006.01) C07K 14/36 (2006.01) C12N 15/70 (2006.01) C12N 15/76 (2006.01) C12N 15/77 (2006.01)

Patent

CA 1294900

Abstract of the disclosure: S. ghanaensis DSM 2932 is resistant to gentamicin at up to 20 µg/ml. Total digestion of the genomic DNA with BglII, incorporation of the restriction fragments into a suitable plasmid, and selection using gentamicin results in gentamicin-resistant clones which contain a 7 kb frag- ment with the gentamicin-resistance gene. The plasmid pPH1JI likewise contains a gentamicin-resistance gene located on a 2.3 kb HindIII-BamHI fragment. These genes are suitable as markers, in particular for Streptomycetes vectors.

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