Geobacillus stearothermophilus alpha-amylase (amys) variants...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/28 (2006.01) C11D 3/386 (2006.01)

Patent

CA 2726265

Described are variants of a parent alpha-amylase that exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal sta-bility, pH-dependent activity, pH-dependent stability, oxidative stability, calcium dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desiz-ing, and/or sweetener production.

L'invention porte sur des variants d'une alpha-amylase parente qui présente une altération d'au moins l'une des propriétés suivantes par rapport à ladite alpha-amylase parente : activité spécifique, spécificité de substrat, liaison au substrat, clivage du substrat, stabilité thermique, activité dépendante du pH, stabilité dépendante du pH, stabilité à l'oxydation, dépendance du calcium, pI et performance de lavage. Les variants sont appropriés pour la conversion de l'amidon, la production d'éthanol, le lavage de la lessive, le lavage de la vaisselle, le nettoyage de surfaces dures, le désencollage de textiles et/ou la production d'édulcorants.

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