Geranyl diphosphate synthase large subunit, and methods of use

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C07K 14/415 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/13 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2387734

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl disphosphate synthase large subunit. In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthaxe large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

Selon l'invention, un ADNc codant pour une sous-unité de grande dimension de géranyl diphosphate synthétase de menthe poivrée, est isolé et séquencé, et la séquence d'acide aminé correspondante déterminée. Des véhicules de clonage recombinant réplicables codent pour la sous-unité de grande dimension de géranyl diphosphate synthétase. Dans un autre mode de réalisation, l'invention concerne des cellules hôtes modifiées qui ont été transformées, transfectées, infectées et/ou injectées avec un véhicule de clonage recombinant et/ou une séquence d'ADN codant pour la sous-unité de grande dimension de géranyl diphosphate synthétase. Dans un autre mode de réalisation encore, la présente invention porte sur une protéine isolée de géranyl diphosphate synthétase de recombinaison, constituée d'une sous-unité de protéine de grande dimension, ainsi qu'une sous-unité de protéine de petite dimension, toutes deux isolées. Ainsi, l'invention fournit des systèmes et des procédés d'expression de recombinaison de géranyl diphosphate synthétase.

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