Germ extract for cell-free protein synthesis and process for...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C12N 9/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2474466

It is intended to provide a process for producing a plant germ extract wherein the step of breaking a plant germ into fine pieces is carried out by impacting or cutting and/or in the presence of an extraction solvent, and a germ extract obtained by this process which is contaminated with little impurities unnecessary or exerting undesirable effects in cell-free protein synthesis. Thus, cell-free protein synthesis can be performed at a high stability and a high efficiency.

L'invention concerne un procédé permettant de produire un extrait de germes végétaux. Dans ce procédé, l'étape de fractionnement du germe végétal en fractions fines, est effectuée par projection ou hachage, éventuellement en présence d'un solvant d'extraction. L'extrait de germes obtenu au moyen de ce procédé présente un faible taux de contamination par des impuretés superflues, ou des impuretés produisant des effets indésirables lors de la synthèse acellulaire de protéines. Ce procédé permet par conséquent une synthèse acellulaire de protéines présentant une stabilité et une efficacité élevées.

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