Gibberellin 2-oxidase gene, functions and uses thereof

Details Gibberellin 2-oxidase gene, functions and uses thereof Gibberellin 2-oxidase gene, functions and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C07H 21/04 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2422599

An objective of the present invention is to provide DNAs encoding novel plant proteins having a gibberellin (GA) 2-oxidation activity. Another objective is to modify plant height by utilizing these DNAs for regulating the gibberellin content. PCR was performed using degenerate primers, and novel OsGA2ox2 and OsGA2ox3 genes were obtained. The present inventors discovered that unlike products of other GA2-oxidases such as OsGA2ox1, the product of OsGA2ox3 catalyzes the two-step oxidation of GA20 to GA29 and then of GA29 to GA29-catabolite. In addition, the present inventors also found that the growth of the transgenic rice plants expressing OsGA2ox3 was suppressed as compared to control plants.

La présente invention a pour objet des ADN codant de nouvelles protéines végétales ayant une activité d'oxydation en position 2 de la gibbérelline (GA). La présente invention a aussi pour objet la modification de la hauteur de végétaux en utilisant ces ADN pour réguler la teneur en gibbérelline. Une PCR a été réalisée au moyen d'amorces dégénérées, et de nouveaux gènes OsGA2ox2 et OsGA2ox3 ont ainsi été obtenus. Les inventeurs de la présente ont trouvé que, contrairement aux produits d'autres GA-2 oxydases, par exemple l'OsGA2ox1, le produit de l'OsGA2ox3 catalyse l'oxydation en deux étapes des GA20 en GA29, puis ensuite celle de GA29 en un catabolite de GA29. De plus, les inventeurs de la présente ont aussi trouvé que la croissance des plants transgéniques de riz exprimant l'OsGA2ox3 était supprimée comparativement à celle de végétaux témoins.

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