Glimepiride- and insulin-induced...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/02 (2006.01) C07F 9/6571 (2006.01)

Patent

CA 2556868

The present invention refers to a method for identification of a chemical compound which modulates the activity of mammalian GPI-PLC wherein a] a mammalian cell is incubated with glimepiride; b] hcDlGs of the cells of a] are prepared; c] the hcDlGs from b] are incubated with a chemical compound; d] the activity of the GPI-PLC from the hcDlGs of c] is determined.

L'invention concerne un procédé d'identification d'un composé chimique qui module l'activité d'une GPI-PLC mammifère. Ce procédé comprend a] l'incubation d'une cellule mammifère avec glimépiride, b] la préparation de hcDIG des cellules de a], c] l'incubation des hcDIG de b] avec un composé chimique et d] la détermination de l'activité de la GPI-PLC à partir des hcDIG de c].

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