C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 15/31 (2006.01) C12P 19/28 (2006.01) C12P 19/30 (2006.01)
Patent
CA 2392000
A polypeptide having an N-acetylglucosamine-1-phosphate uridyltransferase (hereinafter abbreviated as GlmU) activity; a DNA encoding this polypeptide; a recombinant DNA containing this DNA; a transformant carrying this recombinant DNA; a process for producing the GlmU polypeptide by using this transformant; and a process for producing uridine 5'-diphosphate-N-acetylglucosamine by using this transformant. Thus, the GlmU polypeptide originating in a microorganism belonging to Corynebacterium glutamicum can be produced on a mass scale by gene recombination techniques. By using this enzyme, moreover, uridine 5'-diphosphate-N-acetylglucosamine can be efficiently produced.
L'invention concerne un polypeptide présentant une activité de N-acétylglucosamine-1-phosphate uridyltransférase (ci-après appelée GlmU), un ADN codant pour ce polypeptide, un ADN recombinant contenant cet ADN, un transformant porteur de cet ADN recombinant, une méthode de production de ce polypeptide GlmU au moyen de ce transformant, ainsi qu'une opération destinée à la production de 5'-diphosphate-N-acétylglucosamine au moyen dudit transformant. Ainsi, le polypeptide GlmU provenant d'un micro-organisme appartenant à l'espèce Corynebacterium glutamicum peut être produit à grande échelle grâce à des techniques de recombinaison génétique. De plus, l'utilisation de cette enzyme permet de produire efficacement de l'uridine 5'-disphosphate-N-acétylglucosamine.
Nagai Kazuo
Wachi Masaaki
Goudreau Gage Dubuc
Kyowa Hakko Kogyo Co. Ltd.
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