Global amplification of nucleic acids

Details Global amplification of nucleic acids Global amplification of nucleic acids

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2233228

Nucleic acid starting material composed of a collection of single stranded nucleic acid molecules is anchored and processed by a directional random priming method to produce a mixture of shorter random size DNA molecules well- suited for achieving substantially uniform global PCR amplification. The processing and global amplification method disclosed is especially useful in conjunction with subtractive hybridization procedures applied, for example, to the study of differential gene expression.

L'invention concerne la fixation et le traitement par un procédé d'amorçage aléatoire dirigé d'un produit de départ d'acide nucléique composé d'un ensemble de molécules d'acide nucléique à brin unique, ce qui permet de produire un mélange de molécules d'ADN de taille aléatoire plus courte tout indiqué pour effectuer une amplification en chaîne par polymérase globale sensiblement uniforme. Le procédé de traitement et d'amplification globale selon l'invention est notamment utile, conjointement avec des procédés d'hybridation par soustraction, par exemple, dans l'étude de l'expression génique différentielle.

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