Glucagon-like-peptide-2 (glp-2) analogues

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/16 (2006.01) A61K 38/26 (2006.01) A61K 48/00 (2006.01) A61P 1/00 (2006.01) A61P 5/00 (2006.01) C07K 14/605 (2006.01)

Patent

CA 2607150

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and/or improved chemical stability, e.g. as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and/or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and/or a deletion of one or more of amino acids 31 to 33 and/or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy.

L'invention concerne des analogues du GLP-2 qui comportent une ou plusieurs substitutions par comparaison à [hGly2]GLP-2, et qui présentent une activité biologique améliorée in vivo et/ou une stabilité chimique améliorée, évaluée p. ex. par des tests de stabilité in vitro. De manière plus spécifique, les analogues de GLP-2 préférés, comprennent des substitutions dans une ou plusieurs des positions 8, 16, 24 et/ou 28 de la séquence GLP-2 de type sauvage, éventuellement en combinaison avec d'autres substitutions en position 2 (telles que mentionnées dans l'introduction), et dans une ou plusieurs des positions 3, 5, 7, 10 et 11, et/ou une délétion d'un ou de plusieurs des acides aminés 31 à 33, et/ou l'addition d'une séquence peptidique de stabilisation N-terminale ou C-terminale. Ces analogues conviennent particulièrement pour la prévention ou le traitement des troubles gastro-intestinaux et pour l'amélioration des effets secondaires de la chimiothérapie.

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