C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/00 (2006.01) C12P 19/04 (2006.01)
Patent
CA 2761257
The invention relates to the field of poly- and oligosaccharides and their nutritional effects. In particular, it relates to the application of a-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (a1?6) glucosidic linkages and one or more consecutive (a1?4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (a1?4) linked D-glucose units with an ?-glucanotransferase capable of cleaving (a1?4) glucosidic linkages and making new (a1?4) and (a1?6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.
L'invention porte sur le domaine des poly et oligosaccharides et sur leurs effets nutritionnels. En particulier, l'invention porte sur l'application d'a-glucanotransférases dans des procédés de préparation de fibres alimentaires, comprenant des oligosaccharides prébiotiques et sur de nouveaux oligosaccharides pouvant être obtenus par ceux-ci. L'invention porte sur un procédé de production d'un mélange de gluco-oligosaccharides ayant une ou plusieurs liaisons glucosidiques (a1?6) consécutives et une ou plusieurs liaisons glucosidiques (a1?4) consécutives, comprenant la mise en contact d'un substrat de poly et/ou oligosaccharidique comprenant au moins deux unités de D-glucose liées en (a1?4) avec une a-glucanotransférase capable de cliver les liaisons glucosidiques (a1?4) et de créer de nouvelles liaisons glucosidiques (a1?4) et (a1?6). L'invention porte également sur des gluco-oligosaccharides (isolés) pouvant être obtenus ainsi, et sur leur application dans des compositions nutritionnelles et cosmétiques.
Dijkhuizen Lubbert
Dobruchowska Justyna Malgorzata
Kamerling Johannis Paulus
Kralj Slavko
Leemhuis Reinder Johannes
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Rijksuniversiteit Groningen
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