Glucoamylase variants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/34 (2006.01) C07K 14/37 (2006.01) C12N 15/31 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2702019

The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.

La présente invention concerne des variants de glucoamylase. En particulier, l'invention concerne des variants dans le domaine de liaison à l'amidon (SBD) d'une glucoamylase. L'invention concerne aussi des variants ayant des propriétés modifiées (par exemple une meilleure stabilité thermique et/ou une activité spécifique accrue) par rapport à une glucoamylase parente correspondante. La présente invention concerne également des compositions enzymatiques contenant les variants de glucoamylase; des constructions d'ADN contenant des polynucléotides codant pour les variants; et des procédés de production des variants de glucoamylase dans des cellules hôtes.

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