C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/00 (2006.01) C12N 15/74 (2006.01) C12P 19/02 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2370467
The invention relates to the fields of molecular biology, bacteriology and industrial fermentation. More specifically, the invention provides isolated nucleic acid molecules encoding the three subunits of a novel, membrane-bound, Gluconobacter oxydans sorbitol dehydrogenase (SDH) of the invention and vectors and host cells containing said isolated nucleic acid molecules. The invention further provides isolated polypeptides for the three subunits of the SDH enzyme of the invention, and processes for the production of L-sorbose and 2-keto-L-gulonic acid.
L'invention concerne les domaines de la biologie moléculaire, de la bactériologie et de la fermentation industrielle. Plus spécifiquement, l'invention concerne des molécules d'acides nucléiques isolées qui codent pour les trois sous-unités d'une nouvelle sorbitol déshydrogénase membranaire (SDH) issue de Gluconobacter oxydans, ainsi que des vecteurs et des cellules hôtes contenant lesdites molécules d'acides nucléiques isolées. L'invention concerne en outre les polypeptides isolés des trois sous-unités de l'enzyme SDH de l'invention, et des procédés de production de L-sorbose et d'acide 2 céto-L-gulonique.
Choi Eui-Sung
Lee Eun-Hae
Rhee Sang-Ki
Archer-Daniels-Midland Company
Goudreau Gage Dubuc
Komitech
Korea Research Institute Of Bioscience And Biotechnology
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