Glucose dehydrogenase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12M 1/40 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/04 (2006.01) C12N 15/63 (2006.01) C12Q 1/32 (2006.01) C12Q 1/54 (2006.01)

Patent

CA 2491627

It is intended to disclose a modified glucose dehydrogenase characterized in that, in glucose dehydrogenase accompanied by pyrroloquinoline quinone as a coenzyme, one or more amino acid residues are substituted by other amino acid residues in the region of from the 186- to 206-residues in water-soluble PQQGDH originating in Acinetobacter caloaceticus or an equivalent region of another species. It is also intended to provide a gene encoding the modified glucose dehydrogenase as described above, a vector containing the gene, a transformant containing the gene, and a glucose assay kit and a glucose sensor containing the modified glucose dehydrogenase as described above.

L'invention concerne une glucose déshydrogénase modifiée qui se caractérise en ce que, dans la glucose déshydrogénase accompagnée de pyrroloquinoléine quinone en tant que co-enzyme, un ou plusieurs restes d'acide aminé sont substitués par d'autres restes d'acide aminé dans la zone des résidus 186 à 206 de PQQGDH hydrosoluble provenant d'Acinetobacter caloaceticus ou une zone équivalente d'autres espèces. L'invention porte également sur un gène codant pour la glucose déshydrogénase modifiée susdécrite, sur un vecteur contenant le gène, sur un transformant contenant le gène et sur un kit de dosage de glucose ainsi que sur un détecteur de glucose contenant ladite glucose déshydrogénase.

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