Glucose dehydrogenase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12M 1/34 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 15/63 (2006.01) C12Q 1/32 (2006.01)

Patent

CA 2372741

A modified glucose dehydrogenase characterized in that, in water soluble glucose dehydrogenase accompanied by pyrrolo-quinoline quinone as a coenzyme, one or more amino acid residues in a specific region has been substituted by other amino acid residue(s). This modified PQQGDH has an improved affinity for glucose.

L'invention concerne une glucose-déshydrogénase modifiée qui se caractérise en ce que, dans une glucose-déshydrogénase hydrosoluble accompagnée de quinone de pyrrolo-quinoléine en tant que co-enzyme, un ou plusieurs restes d'acide aminé dans une zone spécifique ont été substitués par un ou plusieurs restes d'acide aminé. Ladite PQQGDH modifiée présente une affinité améliorée pour le glucose.

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