Glucose dehydrogenase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 9/04 (2006.01) C12P 21/02 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2461755

A modified water-soluble glucose dehydrogenase which acts with pyrroloquinoline quinone as a coenzyme and in which one or more amino acid residues present on the surface of the enzyme molecule and present in a region which is thought to have side chains exposed on the molecular surface and not interacting with other residues and is thought to be neither an enzymatically active area nor a substrate-bonding area have been replaced with arginine. The one or more amino acid residues are preferably selected from the group consisting of glutamine, asparagine, and threonine. This modified enzyme can be efficiently recovered after production by recombination.

L'invention concerne un déshydrogénase de glucose modifié soluble dans l'eau, qui agit avec la pyrroloquinoline quinone en tant que coenzyme et dans lequel on a remplacé, par de l'arginine, au moins un résidu d'amino-acide présent sur la surface de la molécule enzymatique et dans une zone estimée avoir des chaînes latérales exposées sur la surface moléculaire et n'interagissant pas avec d'autres résidus, et estimée ne pas être une zone active de manière enzymatique ou une zone de liaison de substrat. Lesdits résidus d'amino-acide sont de préférence choisis dans le groupe formé de glutamine, d'asparagine et de thréonine. L'enzyme modifié peut être récupéré de façon efficace après production par recombinaison.

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