Glucose isomerase immobilized on porous phenolic resin...

C - Chemistry – Metallurgy – 12 – N

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195/35.4

C12N 9/92 (2006.01) C12N 11/08 (2006.01) C12P 19/24 (2006.01)

Patent

CA 1134765

3218 GLUCOSE ISOMERASE IMMOBILIZED ON POROUS PHENOLIC RESIN SUPPORT AND METHOD EMPLOYING SAME ABSTRACT OF THE DISCLOSURE An immobilized system comprising glucose isomerase immobilized on a porous phenolic resin having a total surface area of about 40 to about 200 square meters per gram in the range of pores above about 30A° of which at least 30% of the pores have a radius of about 30 to about 250A° and a water swelling capacity of at least 10% by volume and wherein said system has a binding efficiency of at least 90% when an enzyme load of 500 U/ml carrier is offered or at least 80% when an enzyme load of 1000 U/ml carrier is offered, and an enzyme half-life during isomerization of at least 10 days. Glucose is converted into levulose using this system.

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