Glucose oxidase immunohistochemical detection of antinuclear...

Details Glucose oxidase immunohistochemical detection of antinuclear... Glucose oxidase immunohistochemical detection of antinuclear...

G - Physics

01

N

167/45

G01N 33/543 (2006.01) G01N 33/552 (2006.01) G01N 33/567 (2006.01)

Patent

CA 1206092

-1- GLUCOSE OXIDASE IMMUNOHISTOCHEMICAL DETECTION OF ANTINUCLEAR ANTIBODIES ABSTRACT For use in the immunohistochemical detec- tion of antigens and related antibodies in a test serum, an antigen source fixed on a support and stabilized by dehydration treatment with an organic solvent mixture, such as acetone methanol, or mixtures thereof. The support is stored under nitrogen in a sealed polyethylene bag. Further, an immunoenzymatic method for the detec- tion of biological components for a test sample, the method comprising the steps of: contacting the stabilized antigen source with the test sample, contacting the antigen source with an antibody conjugated with glucose oxidase and reactive with the component; incubating the antigen souce with a solution containing glucose and a chromagenic mixture; and analyzing the antigen source for the presence of color, preferably with a light microscope.

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