Glucose-regulated promoter of yeast acetyl-coa hydrolase

Details Glucose-regulated promoter of yeast acetyl-coa hydrolase Glucose-regulated promoter of yeast acetyl-coa hydrolase

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 9/16 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2098463

Abstract of the Invention This invention is directed to the acetyl-CoA hydrolase enzyme of yeast and to a method for purifying acetyl-CoA hydrolase. The enzyme has a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 units wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity and 1 unit of acetyltransferase activity is defined as the amount of enzyme needed to transfer 1 pmol of [3H] acetyl group from [3H] acetyl coenzyme A to ACTH (1-24) in the acetyltransferase standard enzyme assay under standard conditions. The invention is further directed to the glucose-repressible promoter which regulates the expression of the acetyl-CoA hydrolase gene in yeast, to vectors incorporating this promoter and to host cells transformed withsuch vectors.

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