Glucose responsive .beta.-cell line

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 5/06 (2006.01) A61K 35/12 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2302830

The present invention provides an immortalized insulin producing human .beta.- cell which may be rendered glucose responsive by suitable bioengineering methods. The invention also provides a method for producing an immortalized glucose responsive insulin producing human .beta.-cell comprising the steps of selecting an unregulated immortalized human insulin secreting .beta.-cell, transfecting said selected cell line with elements for the genetic control of glucose responsiveness and proliferating said transfected .beta.-cell accordingly.

La présente invention concerne une insuline immortalisée produisant des cellules .beta. humaines qui peuvent être rendues sensibles au glucose grâce à des procédés de génie biologique appropriés. Cette invention concerne également un procédé permettant de produire une insuline sensible au glucose immortalisée produisant des cellules .beta. humaines. Ce procédé consiste à sélectionner une insuline humaine immortalisée non régulée sécrétant des cellules .beta.; à transfecter ladite lignée cellulaire sélectionnée avec des éléments permettant de commander génétiquement la sensibilité au glucose; et à faire proliférer lesdites cellules .beta. transfectées de manière appropriée.

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