Glucose responsive insulin secreting beta-cell lines and...

Details Glucose responsive insulin secreting beta-cell lines and... Glucose responsive insulin secreting beta-cell lines and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) A61K 9/50 (2006.01) A61K 9/66 (2006.01) A61K 35/39 (2006.01) A61L 27/38 (2006.01) A61M 37/00 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12Q 1/54 (2006.01) G01N 33/50 (2006.01) A61K 35/12 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2178621

The invention pertains to a method of selecting cells which have enhanced secretion of secretory products by exposing a population of cells to a secretagogue in concentrations sufficient to form the secretory products. In particular, the method involves the selection of insulin-secreting beta-cells which have been exposed to the secretagogue, glucose, at an effective concentration. The selection process is accomplished by employing a fluorescence-activated cell sorter for the cells which have been marked with a calcium-activated labelling agent.

L'invention se rapporte à un procédé de sélection de cellules à sécrétion améliorée de produits sécrétoires, et consistant à exposer une population de cellules à un sécrétagogue en concentrations suffisantes pour former les produits sécrétoires. Ce procédé consiste plus particulièrement à sélectionner des cellules bêta à sécrétion d'insuline qui ont été exposées au sécrétagogue glucose en une concentration efficace. Le processus de sélection est accompli par l'utilisation d'un trieur de cellules activé par fluorescence pour les cellules qui ont été marquées au moyen d'un agent de marquage activé par calcium.

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