Glucose transport mutants for production of biomaterial

Details Glucose transport mutants for production of biomaterial Glucose transport mutants for production of biomaterial

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/31 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2500741

A method is disclosed for restoring a Glu+ phenotype to a PTS.macr./Glu.macr. bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.

L'invention concerne un procédé destiné à restaurer un phénotype Glu?+¿ en une cellule bactérienne PTS?-¿/Glu?-¿ qui était capable à l'origine d'utiliser un système de transport de phosphotransférase (PTS) pour le transport de carbohydrate. Des cellules batériennes comprenant le phénotype Glu?+¿ ont modifié leurs zones régulatoires chromosomiques endogènes qui sont liées fonctionnelles au polynucléotides codant des perméases galactoses et des glucokinases.

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