Glycoprotein and process for isolating it

Details Glycoprotein and process for isolating it Glycoprotein and process for isolating it

C - Chemistry, Metallurgy

07

K

530/15.14, 167/1

C07K 16/18 (2006.01) C07K 14/47 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1110617

Abstract of the Disclosure: A new glycoprotein is characterized by a protein pro- portion essentially consisting of 75 ? 6 % of .alpha.-amino-acids, a carbohydrate proportion of 24.6 ? 5.2 %, of which 8.9 ? 2 % of hexoses, 7.1 + 1.5 of N-acetylated hexosamine, 0.2 ? 0.2 % of fucose, 8.4 ? 1.5 %, N-acetylated neurami- nic acid; a sedimentation coefficient S20w of 2.5 ? 0.3 S; a molecular weight of 35,000 ? 5,000, determined in the ultracentrifuge and a molecular weight of 65,000 ? 10,000 determined in polyacryl amide gel containing sodium dodecyl sulfate; an iso-electric point at pH 3.4 ? 0.4; an extinction coefficient E ?%cm (280 nm) of 1.9 ? 0.3; an electrophoretic mobility in the range between that of .alpha.1 and .alpha.2-globulines; and a specific immunologic reaction with an antibody specifically directed against the glycoprotein.

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