Glycoprotein production method and screening method

Details Glycoprotein production method and screening method Glycoprotein production method and screening method

C - Chemistry, Metallurgy

07

K

C07K 14/00 (2006.01) C07K 1/113 (2006.01) C07K 1/16 (2006.01)

Patent

CA 2734124

Disclosed is a method for producing a glycoprotein which is uniform in terms of functions derived from a sugar chain (e.g., a blood half-life) as well as physiological activities, i.e., a glycoprotein which is uniform in terms of the amino acid sequence, the sugar chain structure and the higher-order structure. Specifically disclosed is a method for producing a glycoprotein which is uniform in terms of the amino acid sequence, the sugar chain structure and the higher-order structure, which comprises the following steps (a) to (c): (a) refolding a glycoprotein which is uniform in terms of the amino acid sequence and the sugar chain structure; (b) fractionating the refolded glycoprotein by column chromatography; and (c) collecting a fraction having a specified activity.

L'invention porte sur un procédé de production d'une glycoprotéine qui est uniforme en termes de fonctions issues d'une chaîne de sucre (par exemple une demi-vie dans le sang) ainsi que d'activités physiologiques, à savoir une glycoprotéine qui est uniforme en termes de séquence d'acides aminés, de structure de chaîne de sucre et de structure d'ordre supérieur. De façon spécifique, l'invention porte sur un procédé de fabrication d'une glycoprotéine qui est uniforme en termes de séquence d'acides aminés, de structure de chaîne de sucre et de structure d'ordre supérieur, qui comprend les étapes suivantes (a) à (c) : (a) replier une glycoprotéine qui est uniforme en termes de séquence d'acides aminés et de structure de chaîne de sucre; (b) fractionner la glycoprotéine repliée par chromatographie sur colonne; et (c) collecter une fraction ayant une activité spécifiée.

Affiliated with

Also associated with

Rating

Glycoprotein production method and screening method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glycoprotein production method and screening method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glycoprotein production method and screening method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1849217