Gntiii (udp-n-acetylglucosamine:beta-d mannoside beta...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A61K 38/02 (2006.01) C12N 9/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2478294

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTII (EC 2.4.1.144)l that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.

La présente invention concerne le traitement des glycoprotéines dans les plantes transgéniques utilisées comme usines économiques et non polluantes pour la production de protéines biopharmaceutiques de recombinaison ou de compositions pharmaceutiques comprenant lesdites glycoprotéines. L'invention concerne une plante contenant une enzyme fonctionnelle de mammifère produisant de la GnTIII de mammifère qui n'est normalement pas présente dans les plantes, ladite plante contenant, de plus, au moins une seconde protéine de mammifère, ou son fragment fonctionnel, qui n'est normalement pas présente dans les plantes.

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