C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01)
Patent
CA 2638897
Methods for generating an expression-enhanced GPR22 nucleic acid, as well as substituted GPR22 nucleic acids providing for enhanced expression of the encoded GPR22 polypeptide, are provided. In practicing the subject methods, a nucleic acid encoding a mammalian GPR22 receptor polypeptide (e.g., a wild- type nucleic acid) is expression-enhanced by identifying the various codons of the coding region for the GPR22 amino acid sequence and substituting nucleotides so as to enhance expression without changing the amino acid sequence of the encoded GPR22 polypeptide. Methods, compositions, and kits using the same for screening of modulators of GPR22 are also provided.
L'invention concerne des méthodes de génération d'un acide nucléique GPR22 amélioré par expression, ainsi que des acides nucléiques GPR22 substitués permettant l'expression améliorée du polypeptide GPR22 codé. Les méthodes de l'invention consistent à améliorer par expression un acide nucléique codant pour polypeptide récepteur du GPR22 mammifère (par exemple un acide nucléique de type sauvage) par identification des divers codons de la zone de codage pour la séquence d'acides aminés GPR22 et substitution des nucléotides de manière à améliorer l'expression sans changer la séquence d'acides aminés du polypeptide GPR22 codé. L'invention concerne également des méthodes, des compositions et des kits comprenant celles-ci pour le criblage des modulateurs de GPR22.
Arena Pharmaceuticals Inc.
Smart & Biggar
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