Gradient system

Details Gradient system Gradient system

G - Physics

01

N

341/38, 342/38

G01N 30/36 (2006.01) G01N 30/34 (2006.01)

Patent

CA 1300718

LIQUID CHROMATOGRAPHIC SYSTEM ABSTRACT OF THE DISCLOSURE To supply a programmed gradient to a high- pressure pump for introduction into a column, a mixer, degasser and accumulator communicates with the high-pressure pump to supply fluid thereto, having a volume no more than 150 times the chamber volume of the high-pressure pump. The mixer, degasser and accumulator includes temperature sensors which sense when the mixer, degasser and accumulator has been emptied to a level where it cannot respond to a demand for fluid from said high- pressure pump and provides a signal to a low- pressure pump which responds by cycling to again fill the mixer, degasser and accumulator. Upon receiving a demand signal, the low-pressure pump fills by drawing fluid from a plurality of fluid sources to compose the gradient being used at that time, with the pump slowing during valve opening and losing so as to avoid cavitation.

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