Grafts made from amniotic membrane; methods of separating,...

Details Grafts made from amniotic membrane; methods of separating,... Grafts made from amniotic membrane; methods of separating,...

A - Human Necessities

61

K

A61K 35/50 (2006.01) A01N 1/02 (2006.01) A61L 27/36 (2006.01) A61L 31/00 (2006.01) A61F 2/14 (2006.01)

Patent

CA 2252758

A method for making, storing and using a surgical graft from human amniotic membrane; the resulting graft; and the storage solution. The amniotic membrane is obtained from human placenta, from which the chorion has been separated. Sheets of the amniotic membrane are cut to size and mounted on filter paper. The cells of the amniotic membrane are killed, preferably while being frozen and thawed in the storage solution. The storage solution comprises a culture medium and a hyperosmotic agent, wherein the hydration of the amniotic membrane is maintained. The membrane can be impregnated with therapeutic agents, prior to storage, for use in post surgical healing or other therapies.

Cette invention se rapporte à un procédé servant à fabriquer, à stocker et à utiliser une greffe chirurgicale produite à partir d'une membrane amniotique à la greffe qui en résulte; et à la solution de stockage utilisée. La membrane amniotique est produite à partir de placenta humain, dont le chorion a été séparé. Des lamelles de cette membrane amniotique sont découpées à la grandeur voulue et montée sur un papier filtre. Les cellules de la membrane amniotique sont détruites de préférence en étant congelées et décongelées dans la solution de stockage. Cette solution de stockage contient un milieu de culture et un agent hyperosmotique, dans lequel l'hydratation de la membrane amniotique est maintenue. Ladite membrane peut être imprégnée d'agents thérapeutiques, avant le stockage pour être utilisée dans un processus de cicatrisation post-chirurgicale ou dans d'autres thérapies.

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