Grapevine leafroll virus (type 2) proteins and their uses

Details Grapevine leafroll virus (type 2) proteins and their uses Grapevine leafroll virus (type 2) proteins and their uses

C - Chemistry, Metallurgy

12

N

C12N 15/02 (2006.01) C07K 14/005 (2006.01) C07K 14/01 (2006.01) C07K 14/08 (2006.01) C07K 16/08 (2006.01) C12N 15/11 (2006.01) C12N 15/40 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2290551

The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

L'invention porte sur des protéines ou polypeptides isolés à partir du virus (de type 2) de la nécrose réticulée de la vigne, sur les molécules codeuses d'ADN soit seules sous une forme isolée, soit dans un système d'expression, sur une cellule hôte ou sur un plant transgénique de vigne. D'autres aspects de l'invention portent sur un procédé conférant la résistance à la nécrose réticulée de la vigne à des plants de vigne et de tabac en les transformant à l'aide des molécules d'ADN de l'invention, sur un procédé conférant la résistance au virus de la jaunisse grave de la betterave à des plants de betteraves, sur un procédé conférant la résistance au virus de la tristeza à des plants d'agrumes, et sur un procédé de détection de la présence du virus de la nécrose réticulée de la vigne tel que le GRLaV-2 dans un échantillon.

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