Growth control for cells encapsulated within bioartificial...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/071 (2010.01) A01K 67/027 (2006.01) A61K 35/12 (2006.01) A61L 27/18 (2006.01) A61L 27/22 (2006.01) A61L 27/38 (2006.01) C07K 14/82 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2195446

This invention relates to methods and compositions of controlling cell distribution within a bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibing compound; exposing the cells to irradiation, and (3) modifying a growth surface of the BAO with ECM molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination.

Cette invention se rapporte à des procédés et à des compositions qui permettent de contrôler la distribution des cellules dans un organe bioartificiel, en exposant les cellules à un traitement qui inhibe la prolifération cellulaire, qui favorise la différenciation cellulaire ou qui affecte la fixation des cellules à une surface de croissance à l'intérieur de l'organe bioartificiel. Ces traitements consistent: (1) à manipuler génétiquement les cellules, (2) à exposer les cellules à un composé inhibiteur de prolifération ou à un composé inducteur de différenciation ou à retirer les cellules de leur exposition à un composé stimulateur de prolifération ou à un composé inhibiteur de différenciation, à exposer lesdites cellules à un rayonnement, et (3) à modifier une surface de croissance de l'organe bioartificiel au moyen de molécules de matrice extra-cellulaire, au moyen de molécules affectant la prolifération cellulaire ou l'adhésion cellulaire, ou au moyen d'un support inerte, ou d'une combinaison de ceux-ci. Ces traitements peuvent être utilisés combinés les uns aux autres.

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