Growth-enhancing media for mycobacteria

Details Growth-enhancing media for mycobacteria Growth-enhancing media for mycobacteria

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) C07C 53/126 (2006.01) C12N 1/38 (2006.01)

Patent

CA 2383397

This invention provides a method for enhancing the growth of acid-fast microorganisms in culture comprising growing the acid-fast microorganisms in the presence of an aliphatic organic acid of 8-10 carbon atoms. The diagnosis of tuberculosis and many other mycobacterial diseases has been hampered by the slow growth of these organisms using currently available culture methods. The addition of octanoic acid to currently available agar and broth media formulations utilized for the cultivation of pathogenic mycobacteria results in a marked reduction in generation time with an increase in growth yield. The growth promoting activity of octanoic acid offers the potential for significantly more rapid diagnosis, and treatment of these diseases.

Cette invention se rapporte à un procédé permettant d'augmenter la croissance de micro-organismes acidorésistants en culture et consistant à faire croître ces micro-organismes acidorésistants en présence d'un acide organique aliphatique de 8 à 10 atomes de carbone. Le diagnostic de la tuberculose et d'un grand nombre d'autres maladies mycobactériennes est gêné par la croissance lente de ces organismes, lorsqu'on utilise les procédés de culture habituellement disponibles. L'addition d'acide octanoïque aux formulations de milieux actuellement disponibles, à base de gélose et de bouillon de culture, utilisées pour la culture des mycobactéries pathogènes, entraîne une réduction sensible du temps de production, accompagnée d'un accroissement du rendement de croissance. L'activité de stimulation de la croissance, déployée par l'acide octanoïque, offre la possibilité d'un diagnostic et d'un traitement considérablement plus rapides de ces maladies.

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