Growth medium for c. histolyticum without ingredients of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) C12N 9/52 (2006.01)

Patent

CA 2668353

The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferablyplant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.

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