Growth of adult pancreatic islet cells

Details Growth of adult pancreatic islet cells Growth of adult pancreatic islet cells

A - Human Necessities

61

K

A61K 35/39 (2006.01) C07K 14/78 (2006.01) C12P 21/00 (2006.01) A61K 35/12 (2006.01) A61K 38/00 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2233859

A method of increasing the number of adult pancreatic islet cells available for transplantation by contacting the cells with laminin 5 extracellular matrix. When contacted with the deposited matrix produced by 804G rat bladder carcinoma cells, a 1,500 fold increase in cell number is observed after three passages in culture. Islet cell expansion also occurs when cells are contacted with 804G soluble matrix. The expanded islet cells contain insulin and respond to glucose challenge.

L'invention concerne un procédé qui permet d'augmenter le nombre des cellules adultes des îlots de Langerhans du pancreas, aux fins de transplantation, et consiste à mettre en contact ces cellules avec une matrice extracellulaire de laminine 5. Après trois passages en culture, le nombre des cellules est multiplié par 1500 suite au contact avec la matrice déposée provenant de cellules d'un carcinome de vessie de rat de type 804G. On observe également une augmentation du nombre des cellules des îlots au contact d'une matrice soluble de type 804G. Les nouvelles cellules des îlots de Langerhans du pancreas ainsi obtenues contiennent de l'insuline et réagissent à l'épreuve de tolérance au glucose.

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