Growth-stimulating polypeptides for use in fish and crustaceans

Details Growth-stimulating polypeptides for use in fish and crustaceans Growth-stimulating polypeptides for use in fish and crustaceans

C - Chemistry, Metallurgy

07

K

C07K 14/46 (2006.01) A23K 1/165 (2006.01) C07K 14/61 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2615833

The invention relates to polypeptides which demonstrate an activity greater than tilapia growth hormone. The aforementioned polypeptides can stimulate the growth, survival and quality of fish and crustacean larvae. In addition, the polypeptides increase the defence of the organism against pathogenic agents and stimulate parameters relating to the immune system of aquatic organisms. The genes that code for said polypeptides were cloned in a Pichia pastoris expression vector which can be used to obtain extracellular protein. The culture supernatant containing the polypeptides in question was administered to aquatic organisms using immersion baths or as a feed supplement.

La présente invention concerne des polypeptides qui montrent une activité supérieure à celle de l'hormone de croissance de tilapia. Ces polypeptides sont capables de stimuler la croissance, la survie et la qualité des larves de poissons et de crustacés. De plus, ils augmentent la défense de l'organisme contre les agents pathogènes et ils stimulent les paramètres relatifs au système immun d'organismes aquatiques. Les gènes qui codent lesdits polypeptides ont été clonés dans un vecteur d'expression de Pichia pastoris qui permet l'obtention de la protéine de manière extracellulaire. Le surnageant de culture qui contient les polypeptides d'intérêt a été administré par bains d'immersion ou sous la forme d'un supplément dans l'aliment à des organismes aquatiques.

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