Gtp binding protein and method for production thereof

Details Gtp binding protein and method for production thereof Gtp binding protein and method for production thereof

C - Chemistry, Metallurgy

12

N

530/13, 530/15.1

C12N 15/12 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01)

Patent

CA 1335431

The present invention provides a GTP binding protein containing the amino acid sequence Thr-Ile-Glu- Asp-Ser-Tyr, with a molecular weight of about 22 K dalton and having GTP binding activity which is inhibited by N-ethyl-maleimide and GTP hydrolysing activity, and a method for the production of a GTP binding protein, which comprises introducing a DNA fragment containing DNA that encodes the GTP binding protein into a cloning site present downstream to a promoter of an expression vector, then introducing the expression vector thus constructed into a host, culturing said host, thereby expressing and accumulating the GTP binding protein and then collecting thereof.

600977

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