Haemolytic saponin extraction and purification method

Details Haemolytic saponin extraction and purification method Haemolytic saponin extraction and purification method

C - Chemistry, Metallurgy

07

J

C07J 63/00 (2006.01) C07H 1/08 (2006.01) C07H 15/10 (2006.01) C07H 15/256 (2006.01)

Patent

CA 2167075

A saponoside mixture is immersed in an absolute ethanol bath at a temperature of 40-60 DEG C; a first rapid hot filtering step is performed in the bath at 40-60 DEG C to separate the polar and non-polar impurities from the dissolved saponins; the filtrate is cooled to cause precipitation thereof; a second filtering step is performed; and the saponin precipitate is dried in a vacuum oven at less than 40 DEG C.

Le mélange saponosidique est plongé dans un bain d'éthanol absolu à la température de 40° à 60 °Celsius; le bain est soumis à une première filtration lapide à chaud (40° à 60°) pour séparer les impuretés polaires et apolaires des saponines dissoutes, le filtrat est précipité par refroidissement; il est procédé à une deuxième filtration puis le précipité constitué par les saponines est séché en étuve sous vide à moins de 40° Celsius.

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