Haptene assay by a competition-based method

Details Haptene assay by a competition-based method Haptene assay by a competition-based method

G - Physics

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G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2208562

Method of assaying haptene in a sample, in which said sample is placed in contact with antibodies capable of recognizing haptene and with a predetermined quantity of a reagent capable of competing with said haptene-fixing antibodies. The reagent is a conjugate formed by a single-stranded fragment of nucleic acid with a compound capable of being recognized by said haptene-recognizing antibodies. The fixed or unfixed reactant is detected by antibodies capable of recognizing the nucleic acid fragment of said conjugate. Detection of said reactant by anti-fragment antibodies of said nucleic acid enhances, in particular, the sensitivity of the assay.

Procédé pour le dosage d'un haptène dans un échantillon, dans lequel on met en contact l'échantillon avec des anticorps capables de reconnaître l'haptène et avec une quantité prédéterminée d'un réactif capable d'entrer en compétition avec ledit haptène pour la fixation sur lesdits anticorps. Le réactif est un conjugué formé par un fragment d'acide nucléique simple brin avec un composé capable d'être reconnu par les anticorps reconnaissant l'haptène. On détecte ledit réactif, fixé ou non fixé, à l'aide d'anticorps capables de reconnaître ledit fragment d'acide nucléique du conjugué. La détection dudit réactif à l'aide des anticorps anti-fragment d'acide nucléique apporte notamment une amélioration de la sensibilité du dosage.

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