Haptens, tracers, immunogens and antibodies for carbazole...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07D 209/88 (2006.01) C07D 307/91 (2006.01) C07D 405/12 (2006.01) C07D 407/12 (2006.01) C07D 409/12 (2006.01) C07D 409/14 (2006.01) C07F 9/572 (2006.01) C07H 21/00 (2006.01) C07K 14/435 (2006.01) C07K 14/76 (2006.01) C07K 16/44 (2006.01) G01N 33/577 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2166782

Novel tethered hapten intermediated and related conjugates based on carbazole and/or dibenzofuran, as well as methods for making and using such conjugates. Haptens based on the above core structures may be substituted at any position on the aromatic rings with a wide variety of substituents. Using tethered intermediates, immunogens, tracers, solid supports and labeled oligonucleotides are all described; as are methods for using the intermediates to prepare the conjugates, methods of using the conjugates to make and purify antibodies, as assay tracers, and in nucleic acid hybridization assays. Kits containing haptenated oligonucleotides and anti-hapten conjugates are also described.

Nouveaux intermédiaires de haptènes liés et conjugués apparentés à base de carbazole et/ou de dibenzofurane, et procédés pour la production et l'utilisation de ces conjugués. Les haptènes constitués par les structures de noyau ci-dessus peuvent être substitués en une position quelconque sur les cycles aromatiques par une grande variété de substituants. L'invention décrit l'utilisation d'intermédiaires liés, des immunogènes, des traceurs, des supports solides et des oligonucléotides étiquetés, ainsi que des procédés d'utilisation des intermédiaires dans la prépartion de conjugués, des procédés d'utilisation des conjugés dans la production et la purification d'anticorps, comme traceurs dans des dosages et dans des dosages d'hybridation d'acide nucléique. Des matériels contenant des oligonucléotides haptènés et des conjugués anti-haptènes sont également décrits.

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