Haptens, tracers, immunogens and antibodies for immunoassays...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/44 (2006.01) C07C 219/06 (2006.01) C07C 229/08 (2006.01) C07C 271/10 (2006.01) C07K 2/00 (2006.01) C09B 57/00 (2006.01) G01N 33/533 (2006.01) G01N 33/566 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2114327

Disclosed is a substantially optically pure hapten, useful in an immunoassay for dextropropoxyphene and/or nordextrop- ropoxyphene. The hapten corresponds to a specified structural formula (IX). Also disclosed is an immunogen derived from the hapten as well as an antibody raised in response to an immunogen derived from the hapten. Also disclosed is a fluorescent tracer derived from a substantially optically pure compound corresponding to the hapten, the tracer being useful in an immuoassay for dextropropoxyphene and/or nordextropropoxyphene. Also disclosed is an improved immunoassay for determining dextropro- poxyphene and/or nordextropropoxyphene in a biological sample involving a step of contacting the sample with antibodies raised in response to the immunogen. Also disclosed is a fluorescence polarization immunoassay (FPIA) for determining dex- tropropoxyphene and/or nordextropropoxyphene involving a step of contacting the sample with antibodies raised in response to the immunogen, and/or involving a step of contacting the sample with a fluorescent tracer.

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