Hbv/hcv virus-like particle

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/295 (2006.01) A61P 31/12 (2006.01) C07K 14/02 (2006.01) C07K 14/18 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2392527

Chimeric antigens derived from hepatitis B virus (HBV) and hepatitis C virus (HCV) are described which form virus-like particles when co-expressed with an excess of hepatitis B virus surface antigen (HBsAg). The chimeric antigens are fusion proteins containing an immunogenic peptide derived from an HCV protein coupled to the amino terminus of HBsAg. Also described are nucleic acid constructs and vectors for transfection of cells and expression of the chimeric antigens. The invention further provides methods for producing HBV/HCV virus-like particles containing the chimeric antigens, cell lines for producing the virus-like particles, combination vaccines containing the virus- like particles, and DNA vaccines that express the virus-like particles.

L'invention concerne des antigènes chimères dérivés du virus de l'hépatite B (HBV) et du virus de l'hépatite C (HCV) qui, lorsqu'ils sont coexprimés avec un excès d'antigène de surface de l'hépatite B (HBsAg), forment des particules de type virus. Les antigènes chimères sont des protéines hybrides comprenant un peptide immunogène dérivé d'une protéine du virus de l'hépatite C couplée aux N-terminaux de l'antigène de surface de l'hépatite B. L'invention concerne également des vecteurs et des constructions d'acides nucléiques destinés à la transfection cellulaire et à l'expression des antigènes chimères. L'invention concerne en outre des méthodes de production de particules de type virus HBC/HCV contenant des antigènes chimères; des lignées cellulaires permettant de produire ces particules; des combinaisons de vaccins contenant lesdites particules; et des vaccins à ADN exprimant les particules de type virus.

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